Journal: International Journal of Biological Sciences
Article Title: TRPV1 Downregulation Impairs Prostate Cancer Growth: Functional and Translational Insights from Cellular and In Vivo Models
doi: 10.7150/ijbs.125429
Figure Lengend Snippet: PSA plasma levels and TRPV1 expression in the prostates of TRAMP mice fed different diets. (A) PSA levels in plasma measured by ELISA at the end of the experiment. Results are expressed as mean ± SD (n = 6 per group) along with median values. p -values comparing each group to the STD group are shown in the rightmost column. (B) Left: TRPV1 mRNA levels determined by RT-qPCR, normalized to GAPDH (housekeeping gene) and presented as mean ± SD (n = 6 per group). Right: TRPV1 protein levels assessed by Western blotting with β-actin used as a loading control. A representative blot from two independent samples per group is shown. Densitometric values (mean ± SD) relative to the STD group are presented. (C) Protein levels of PCNA and Cyclin B1, key regulators of proliferation and the cell cycle, analyzed via Western blotting. A representative blot from two samples per group is shown with densitometric values (mean ± SD) relative to the STD group. (D) Relative mRNA expression levels of the stemness markers Oct4 and Nanog in prostate tissues from TRAMP mice. mRNA levels were quantified via RT-qPCR, normalized to GAPDH (housekeeping gene) and presented as mean ± SD (n = 6 per group). (E) Correlation analysis between TRPV1 and the stemness markers Oct4 and Nanog in prostate tissues of TRAMP mice. Top: Scatter plots showing Pearson's correlation coefficients (r) for TRPV1 vs. Oct4 and TRPV1 vs. Nanog. Bottom: Heatmap displaying Pearson's correlation coefficients for TRPV1, Oct4, and Nanog expression. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***) and p < 0.0001 (****) indicate significant differences via one-way ANOVA.
Article Snippet: Plasma levels of prostate-specific antigen (PSA) were measured using a Mouse PSA ELISA Kit (Elabscience®, Houston, TX, USA; catalogue number: E-EL-M0961), following the manufacturer's instructions.
Techniques: Clinical Proteomics, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Control